Fig. 3
Expression of axis-patterning genes in wild-type and bcdCRISPR oocytes. (A) wild-type stage 10 oocyte stained for Hunchback (Hb); both the nurse cell nuclei (NCn) and the germinal vesicle (GV, arrow) exhibit Hb staining. Insert displays a magnification of the GV staining (red) along with DAPI (blue). (B) wild-type stage 10 oocyte stained for Lesswright (Lwr); the position of the germinal vesicle (GV) is indicated with an arrow. Insert features a magnification of the GV staining (red) along with DAPI (blue). (C) wild-type stage 10 oocyte stained for Maelstrom (Mael); the position of the germinal vesicle (GV) is indicated with an arrow. Insert shows a magnification of the GV staining (red) along with DAPI (blue). (D-H) bcdCRISPR ovariole stained for Staufen (D, green), Oskar (E, red), Caudal (F, yellow), DAPI (G, blue) and merge in (H). Posteriorly, Staufen shows partial mislocalization (green arrowheads and arrows in D). Oskar staining at the apices of follicle cells is unspecific staining of the mouse anti Osk antibody. (I-K) bcdCRISPR oocyte string stained for Hb (I, red), DAPI (J, blue) and merge in (K). Hb staining is no longer detectable in the GV (stippled red arrow in I, arrows in GV nucleus (GVN) in J and K), while the nurse cells nuclei still express Hb. (N, O) bcdCRISPR stage 8 oocyte, Lwr (N) and Mael (O) expressions are unaffected in the GV (arrows). The insert between (N) and (O) shows a merge of Lwr, Mael and DAPI staining. All oocytes are oriented anterior to the left. Stages of oogenesis follow the classification of [83]