Fig. 9

Cell migration and invasion ability analysis and Sa-β-gal staining. A Wound healing assay. Magnification × 200. Scale bars, 100 µm. B Quantification of cell migration ability. Wound healing assay showed more higher cell migration ability in the DKC1-deleted cell lines. C Transwell assay. Magnification × 200. D Quantification of cell invasion ability. Transwell assay showed more higher cell invasion ability in the DKC1-deleted cell lines. E SA-β-gal staining (blue). Magnification × 200. F Quantification of SA-β-gal-stained cells. Relatively more Sa-β-gal.⁺ cells were observed in the DKC1-deleted cell lines. Scale bars, 50 µm. * P < 0.05, ** P < 0.01 (Student's t test)