Fig. 7

HMGB1 overexpression promoted tumor proliferation and co-localized with P-gp protein in vivo. (a-b) After cisplatin and paclitaxel therapy, separately, the tumor cell apoptosis was determined using the TUNEL assay. Blue: nucleus; green: cell apoptosis level. Magnification, ×200. (c) Immunofluorescence assay was used to detect co-localization of HMGB1 and P-gp proteins. Blue: nucleus; red: HMGB1 expression; green: P-gp expression. Base magnification: ×200. *P < 0.05, **P < 0.01 and ***P < 0.001