Fig. 2

HMGB1 promoted cisplatin resistance of A549 cells. (a-b) The mRNA and protein expression of HMGB1 in A549 and A549-DDP were detected by qRT-PCR (a) and Western blot (b). (c-d) A549 cells were transfected with NC or HMGB1. Next, western blot was used to determine HMGB1 expression in A549-DDP cells and in transfected A549 cells. qRT-PCR (c) and Western blot (d) were used to determine the interference efficiency of HMGB1. (e-g) IC50 values of paclitaxel, docetaxel, gemcitabine, pemetrexed, and cisplatin, in three cell lines after 24 h, 48 and 72 h treatment, separately. * P < 0.05, ** P < 0.01 and *** P < 0.001