Fig. 6

MCM8 overexpression counteracted the effects of E2F4 knockdown on GSCs traits. A qRT-PCR analysis was used to measure MCM8 expression in OE-ctrl and OE-MCM8 groups. **P-value < 0.01 by one-way analysis of variance, vs. OE-ctrl. B/C Tumor sphere formation of GSCs transfected with si-ctrl, si-E2F4 or co-transfected with si-E2F4 and OE-MCM8 plasmid. **P-value < 0.01 by one-way analysis of variance, vs. si-ctrl. ##P-value < 0.01 and #P-value < 0.05 by one-way analysis of variance, vs. si-E2F4. D/E Limiting dilution assay of GSCs transfected with si-ctrl, si-E2F4 or co-transfected with si-E2F4 and OE-MCM8 plasmid. F/G Relative cell growth of GSCs transfected with si-ctrl, si-E2F4 or co-transfected with si-E2F4 and OE-MCM8 plasmid. **P-value < 0.01 by one-way analysis of variance, vs. si-ctrl. ##P-value < 0.01 by one-way analysis of variance, vs. si-E2F4. H/I Western blot of p-ATR and γH2AX in GSCs transfected with si-ctrl, si-E2F4 or co-transfected with si-E2F4 and OE-MCM8 plasmid