Fig. 5

E2F4 promoted MCM8 transcription by binding to its enhancer. A qRT-PCR analysis was used to measure E2F4 expression in GSCs transfected with si-ctrl or si-E2F4. **P-value < 0.01 by one-way analysis of variance, vs. si-ctrl. B qRT-PCR analysis was used to measure MCM8 expression in GSCs transfected with si-ctrl or si-E2F4. **P-value < 0.01 by Student’s t-test, vs. si-ctrl. C H3K27ac peaks at the MCM8 locus of GSCs and NSCs. The upregulated enhancer regions in GSCs compared to NSCs were marked as region E1 and E2. D/E ChIP-qPCR analysis of anti-E2F4 enrichment at region E1 and E2 in GSCs. **P-value < 0.01 by Student’s t-test, vs. IgG group. F/G ChIP-qPCR was used to detect the enrichment of anti-H3K27ac on the enhancer regions of MCM8 in GSCs transfected with si-ctrl or si-E2F4. **P-value < 0.01 by Student’s t-test, vs. si-ctrl group