Fig. 2

Characterization of enhancer-controlled genes in “activation of ATR in response to replication stress” term. A Differential analysis of H3K27ac signal in GSCs vs. NSCs based on GSE119776 dataset. The cutoff value was |log2 fold change|≥ 1.0 and P-value < 0.05. B Venn diagram showing the intersection of genes with increased enhancer H3K27ac signaling (purple) and upregulated gene enriched in “activation of ATR in response to replication stress” term (yellow). C Comparison of H3K27ac modification levels at the LIN9, MCM8, CEP72, POLA1, DBF4, NDE1 and CDKN2C loci in GSCs vs. NSCs. Statistical analysis was performed using Student’s t-test. D Distribution of H3K27ac peaks at the CDKN2C locus was determined using GSE119776 dataset. Regions with significantly elevated H3K27ac signal in GSCs were labelled as region E1 and E2. E ChIP-qPCR analysis of anti-H3K27ac enrichment of region E1 and E2. **P-value < 0.01 by one-way analysis of variance, vs. hNSCs