Mutations in the acetolactate synthase (ALS) enzyme affect shattercane (Sorghum bicolor) response to ALS-inhibiting herbicides

Table 6 Sequence of primers used to amplify the acetolactate synthase (ALS) sequence from the mutants and wild type biotypes of shattercane from Nebraska

Set	Primer	Nucleotide sequence	Amplified size (bp)
1	Forward	GTGCCCCCGCCCCAAACCCT	1–443
1	Reverse	ACTAGGTTGGTGGCGCCGGG
2	Forward	CACCTCCGGCCCCGGCGCCA	434–1071
2	Reverse	CAATCTTCCCTGTCACACGA
3	Forward	GGTTTGATGATCGTGTGACAGG	1065–1520
3	Reversed	TACGCCCCAAGACCAGCTGAAGA
4	Forward	GCAGTGGTTGTCTTCAGCTGGT	1509–1916
4	Reversed	GATCATAGGCAACACATGATCCT
5	Forward	GTGATGGCAGGACTGTGTAT	1910–2290
5	Reversed	CGTACAGAACCACTGCATAG
6	Forward	TCCGTGTGACAAAGAAGAGCGAA	1800–2280
6	Reversed	GAGGCGTACAGAACCACTGCATAG

ISSN: 1601-5223