Fig. 8
From: Circular RNA circHMCU promotes breast tumorigenesis through miR-4458/PGK1 regulatory cascade
PGK1 overexpression reversed the effects of miR-138-5p mimic on BC cell glycolysis, growth and apoptosis. A qRT-PCR measured miR-4458 expression. B Western blot measured the expression of PGK1. C-J MDA-MB-231 and MCF-7 cells were transfected with mimic NC, miR-4458 mimic, miR-4458 mimic + pcDNA, or miR-4458 mimic + pcDNA-PGK1. C Western blot measured the expression of PGK1. D-F Commerial kits measured the levels of glucose consumption, lactate production and ATP. G-I Cell proliferation was detected by CCK8 and EdU assay. J Cell apoptosis was measured by flow cytometry. *P < 0.05