Fig. 5
From: Circular RNA circHMCU promotes breast tumorigenesis through miR-4458/PGK1 regulatory cascade
Knockdown of miR-4458 reversed the effects of circHMCU on BC cell glycolysis, growth and apoptosis. MDA-MB-231 and MCF-7 cells were transfected with si-NC, si-circHMCU, si-circHMCU + inhibitor NC, or si-circHMCU + miR-4458 inhibitor. A and B qRT-PCR measured miR-4458 expression. C-E Commerial kits were used to measure glucose consumption, lactate production and ATP level. F and G Cell proliferation was detected by CCK8 and EdU assay. H Cell apoptosis was measured by flow cytometry. I Cell invasion was tested by transwell assay. *P < 0.05