Fig. 4
From: Trimeric complexes of Antp-TBP with TFIIEβ or Exd modulate transcriptional activity
BIP2 does not form a trimeric complex with Antp-hTBP by BIFC-FRET. A Schematic representation of BIP2 fused to cyan (ECFP Channel), BiFC by Antp-hTBP interaction (Venus channel), and lack of energy transfer between BIP2 and Antp-hTBP heterodimer (FRET panel). B BIP2 does not form trimers with Antp-hTBP heterodimer (E = 0.14 ± 0.10). C AntpΔN does not modify trimeric complex formation (E = 0.15 ± 0.08). D Antp YPWM mutant (AntpAAAA) does not modify trimeric interaction (E = 0.18 ± 0.14). Color bar represents FRET intensity (Fire mode); darker color indicates low trimeric interaction levels and lighter color indicates high trimeric interaction levels. E The graph shows statistical analysis of three independent triplicates by one-way ANOVA for mean comparison. Error bars correspond to standard error (p ≤ 0.001), ns is not significant. Scale bar, 20 µm