Fig. 3
From: Trimeric complexes of Antp-TBP with TFIIEβ or Exd modulate transcriptional activity
Exd forms a trimeric complex with Antp-hTBP by BiFC-FRET. A Schematic representation of Exd fused to cyan (ECFP Channel), BiFC by Antp-hTBP interaction (Venus channel), and energy transfer due to Antp-hTBP-Exd trimeric complex (FRET). B Exd trimeric interaction with Antp-hTBP heterodimer (E = 0.41 ± 0.23). C Antp-ΔN diminished trimeric complex formation (E = 0.14 ± 0.09). D Antp YPWM mutant (AntpAAAA) affects trimeric interaction (E = 0.21 ± 0.13). Color bar represents FRET intensity (Fire mode); darker color indicates low trimeric interaction levels and lighter color indicates high trimeric interaction levels. E The graph shows statistical analysis of three independent triplicates using a one-way ANOVA for mean comparison, significance is indicated as *** = p ≤ 0.001, ** = p ≤ 0.01, * = p ≤ 0.05 and error bars correspond to standard error (p ≤ 0.001). Scale bar, 20 µm