Fig. 2
From: Trimeric complexes of Antp-TBP with TFIIEβ or Exd modulate transcriptional activity
Trimeric complex formation of Antp-hTBP with TFIIEβ by BiFC-FRET. A Schematic representation of TFIIEβ fused to cyan (ECFP Channel), BiFC by Antp-hTBP interaction (Venus channel), and energy transfer due to Antp-hTBP-TFIIEβ trimeric complex (FRET). B TFIIEβ trimeric interaction with Antp-hTBP heterodimer (E = 0.48 ± 0.25). C AntpΔN diminishes trimeric complex formation (E = 0.17 ± 0.10). D HD deletion of Antp (AntpΔHD) decreases the trimeric complex formation (E = 0.13 ± 0.09). E The Antp helix 2 residues 32 and 36 are important for the trimeric complex (E = 0.17 ± 0.10). Color bar represents FRET intensity (Fire mode); darker color indicates low trimeric interaction levels and lighter color indicates high trimeric interaction levels. F The graph shows statistical analysis of three independent triplicates using a one-way ANOVA for mean comparison, significance is indicated as *** = p ≤ 0.001, ** = p ≤ 0.01, * = p ≤ 0.05 and error bars correspond to standard error (p ≤ 0.001). Scale bar, 20 µm