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Fig. 1 | Hereditas

Fig. 1

From: Inhibition of USP14 suppresses ferroptosis and inflammation in LPS-induced goat mammary epithelial cells through ubiquitylating the IL-6 protein

Fig. 1

LPS stimulates ferroptosis of GMECs. GMECs were divided into 3 groups, one group was treated with LPS, one group was treated with LPS and Fer-1, and the control group was not treated at all. A Cell viability of GMECs was detected by CCK-8 assay. B The secretion of TNF-α was detected by ELISA. C The content of ROS was analyzed with DCFDA-cell ROS detection kit. D MDA content was detected by MDA assay kit. E The GSH levels were detected by GSH assay kit. F The concentration of Fe2+ was measured by using an iron assay kit. G-I The expression level of GPX4 and IL-6 was detected by Western blotting and the grayscale was analyzed by using ImageJ software

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Hereditas

ISSN: 1601-5223

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