Table 1 SNP and qRT-PCR primers of IDO1 and IDO2
Primer | Primer sequences (5′–3′) | PCR purpose |
|---|---|---|
IDO1-Exon2&3 | GAAGGCAAGGCATACTATCAG | SNPs |
GGAAAGTTAAATGTAAATTAGATG | ||
IDO1-Exon4 | CAGGAGCAAGACTCCATCTC | SNPs |
GTAGTGGTAGACACAGCAGTC | ||
IDO1-Exon6 | GATAGTAAGGCCTGCCACAC | SNPs |
GTTTAGGCTCCGAAGTGATTG | ||
IDO1-Exon7 | CTGGACAACTGAGCGAGACTC | SNPs |
CTATTCTACACCTGGAACATTTG | ||
IDO2-R248W | GAACATTCTATCCCCCGTTGC | SNPs |
TTACCTGAGAGTGGATCCCTAGCA | ||
IDO2-Y359X | TCTTGTGCTCCCTCCAAAACA | SNPs |
TGGTTTGGCTTCCCATGCTT | ||
Human IDO1 | GATGTCCGTAAGGTCTTGCCA | qRT-PCR |
TGCAGTCTCCATCACGAAATG | ||
Human IDO2 | CTGATCACTGCTTAACGGCA | qRT-PCR |
TGCCACCAACTCAACACATT | ||
Human β-actin | TGGCACCCAGCACAATGAA | qRT-PCR |
CTAAGTCATAGTCCGCCTAGAAGC | ||
Mouse IDO1 | ATCGC AGCTT CTCCTGCAAT | qRT-PCR |
TGTAT CGATG GTCAGACCTC | ||
Mouse IDO2 | A TA CTG GCC TCT GGT CCT | qRT-PCR |
GGTGGCAGC GGA GAT AAT | ||
Mouse β-actin | AGCCATGTACGTAG CCATCC | qRT-PCR |
CTCTCAGCTGTGGTGGTGAA |