Fig. 9
Analysis of DRx enhancer gene targeting strains. A-D′ Laser confocal images of the anterior parts of stage 16 Drosophila embryos stained using HRP (green) and DRx (red). The anterior ends of the embryos are pointing to the bottom. For each embryo two different focal planes are shown (A, A‘to D, D‘). Expression domains are indicated according to the wild-type (A, A‘). Expression domains with reduced or missing DRx expression in the enhancer gene targeting strains are indicated in yellow. E-G‘Laser confocal images of right hemispheres from third instar larval brains. Again two different focal planes are shown (E, E‘to G, G‘). Staining was performed using Nrt (BP106) (green) and DRx (red). (A, A’) DRx expression in a wild-type embryo is shown as a reference. The expression domains BLD, DAL, DAM, DPLc, DPM, MB reflect those in Fig.1. B, B′ In 77F11KO embryos the DRx expression pattern was similar to that in wild-type embryos. A slight reduction was observed in the central region (yellow arrowhead). C, C′ 77F06KO embryos show less DRx expression in domains DAM and DPM, and expression in domain BLD is missing. (D, D′) The 77F09KO embryos show an altered DRx expression in all domains except the mushroom body domain. Abbreviations: BLD, basal lateral dorsal; DAL, dorsal anterior lateral; DAM, dorsal anterior medial; DPLc, dorsal central lateral; DPM, dorsal posterior medial; MB, mushroom body. E, E’) In the wild-type larval brain, prominent expression of DRx can be observed in the DM and DL lineages (white arrowhead), and weaker expression is seen in the lobula plate (white arrowhead). F, F′ Again, no obvious alterations of the DRx expression pattern were visible compared with the wild-type. G, G’ In strain 77F06KO, the DM lineages looked disorganized and less DRx staining was visible, especially in the upper lineage areas (yellow arrowhead). The DRx expression in the lobula plate was completely gone (yellow arrowhead). Abbreviations: DL, dorsolateral lineages; DM, dorsomedial lineages; LP, lobula plate. (Scale bars: 50 μm)