Fig. 2

Cell type identification of DRx-expressing cells in the larval brain. A-E Larval brains with the central brain regions. A The earmuff R9D11 reporter (R9D11-mCD8-GFP, green) highlights the proximal parts of the six DM lineages (1–6) starting from the INPs. DRx expression (red) is visible in the more central and distal parts of these lineages. B Expression of the earmuff R9D11 reporter (R9D11-mCD8-GFP, green) also highlights the two DL lineages adjacent to the DM lineages. Here, DRx (red) is expressed in more cells of the lineages compared with the neighbouring DM lineages. C Ase expression (blue) marks INPs of type II lineages. Coexpression with DRx (red) was detected in only a few cells, mostly evident in DM6 (white arrowhead). D Expression of DRx (red) in combination with Pros (blue) and Elav (green) indicates DRx expression in GMCs (Pros⁺, Elav⁻, white arrowhead) and neurons (Pros⁺, Elav⁺, yellow arrowhead). E DRx expression (red) in combination with the glial cell marker Repo (green) shows no coexpression. F-I Sections of a larval brain hemisphere focusing on the lobula complex. DRx expression (red) is shown with dll Gal4-mCD8::GFP (green). Abbreviations: DL, dorsolateral lineages; DM, dorsomedial lineages; dIPC, dorsal inner proliferation centre; LP, Lobula plate; NP, neuropile. (Scale bars: 50 μm, 2B 20 μm)