Fig. 1
From: Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting
Generation of the ermKOGal4 strain. Genomic organization of the erm gene with exons indicated as grey boxes. The direction of transcription is shown by an arrow. The location of fragments used for the later analysis of enhancer activities is also indicated (Flybase FB2021_04). Below the genomic organization of four different transcripts (erm-RA to erm-RD) are shown (white untranslated region; blue, translated region). These transcripts are translated into various protein forms using an ATG in exon 2 (erm-PA, erm-PC, erm-PD) or exon 3 (erm-PB). At the bottom is the genomic organization of the ermKOGal4 strain. Here, the region upstream of the ATG in exon 2 up to sequences shortly downstream of the ATG in exon 3 is deleted (black arrowheads) and replaced by Gal4 (yellow) flanked by an attB/P site (red) and a loxP site (green) as shown by [38]