Table 1 The primers using for PCR amplification
NO. | Target | Sequence | TM (°C) |
|---|---|---|---|
1 | ENST00000162749 | F: CTGTACGCCGTGGTGGAGAAC R: TCGTGGTCGCTCAGCCCTA | 60 |
2 | ENST00000398795 | F: ATCAGAGCAGAGAAAGCGATGG R: GATGGGATGTCGGTGGCATTA | 60 |
3 | ENST00000329608 | F: TAGGCAACGAGCGACAGG R: GTAGGGCAGGGTCTCTTAGC | 60 |
4 | ENST00000316804 | F: CTCCGACTCCAGATATGAACAG R: GAGAAGCAGGATGAGGAAGAG | 60 |
5 | ENST00000285379 | F: GCCTCCTTCCTGAATCCTTGG R: CTGATGGGTTCCTTGAGCACAA | 60 |
6 | ENST00000382745 | F: AGAAGGTCGGCGTCATTGTG R: GGCAGGCTGGGTGTCATC | 60 |
7 | ENST00000193322 | F: GGTGGTAAGTGCCTGTAGTCCTA R: GCAGTGGTACAATCTTGGCTCAT | 60 |
8 | hsa-miR-29a-3p | F: CGGCGGTAGCACCATCTG R: GTCGTATCCAGTGCAGGGTCCGAGGTATTC GCACTGGATACGACTAACCG | 60 |
9 | hsa-miR-3165 | F: TCGCAGGTGGATGCAATGT R: GTCGTATCCAGTGCAGGGTCCGAGGTATTC GCACTGGATACGACTGAGGT | 60 |
10 | hsa-miR-410-3p | F: GCGGCGGAATATAACACAGAT R: GTCGTATCCAGTGCAGGGTCCGAGGTATTC GCACTGGATACGACACAGGC | 60 |
11 | hsa-miR-520 h | F: GGCGACAAAGTGCTTCCCT R: GTCGTATCCAGTGCAGGGTCCGAGGTATTC GCACTGGATACGACACTCTA | 60 |
12 | hsa-miR-1226-3p | F: GCGTCACCAGCCCTGTGT R: GTCGTATCCAGTGCAGGGTCCGAGGTATTC GCACTGGATACGACCTAGGG | 60 |
13 | hsa-miR-373-5p | F: TCAAAATGGGGGCGCTT R: GTCGTATCCAGTGCAGGGTCCGAGGTATTC GCACTGGATACGACGGAAAG | 60 |
14 | hsa-miR-1343-3p | F: TCTCCTGGGGCCCGC R: GTCGTATCCAGTGCAGGGTCCGAGGTATTC GCACTGGATACGACGCGAGA | 60 |
15 | GAPDH | F: TGCACCACCAACTGCTTAGC R: GGCATGGACTGTGGTCATGAG | 60 |
16 | U6 | F: CTCGCTTCGGCAGCACA R: AACGCTTCACGAATTTGCGT | 60 |