Fig. 3

Overexpression of mei-41 results in smaller adult tissue size. (a) UAS-mei-41 was overexpressed during eye development using ey-Gal4; likewise was UAS-lacZ serving as control. Eye size of adult female flies was measured as indicated. Compared to the control, mei-41 overexpression caused a significant decrease in eye size (n ≥ 26). (b) Effects on tissue size of the overexpression of UAS-mei-41 with en-Gal4-GFP in the posterior compartment of the developing wing were compared to those of the control UAS-lacZ (n ≥ 20). Size of anterior and posterior compartments was measured as indicated. Whereas no difference was detected in the anterior (a), the posterior compartment (p, shaded grey) was significantly smaller when mei-41 was overexpressed. Longitudinal veins L1-L5 are labelled. Data are depicted as boxplots with center lines showing the medians, box limits indicate the 25th and 75th percentiles as determined by BoxPlotR software; whiskers extend 1.5 times the interquartile range. Statistics were done using ANOVA, and significance determined with Dunnett’s approach (*** p < 0.001)