Fig. 2

Overexpression of mei-41 induces G2/M cell cycle arrest. (a-d) The checkpoint kinases mei-41, grp and lok (chk2) as indicated were overexpressed in the posterior compartment of wing imaginal discs using en-Gal4-GFP (green) (b-d). UAS-lacZ served as control (a). To image M phase, discs were stained with anti-Phospho-Histone H3 (PH3) antibodies (red) indicating the number of mitotic cells. GFP labelling was used to determine the antero-posterior boundary (dotted line). (e) Quantification of PH3 signals showed significant downregulation upon overexpression of mei-41 and grp but not of lok. Significance was tested by ANOVA two-tailed Tukey-Kramer approach (** p < 0.01; * p < 0.05; ns: not significant). Bars represent standard error (SEM) from 13 to 17 analyzed discs per genotype. Size bar represents 100 μm in all panels