Genetic diversity and structure of tea plant in Qinba area in China by three types of molecular markers

Zhang, Yu; Zhang, Xiaojuan; Chen, Xi; Sun, Wang; Li, Jiao

doi:10.1186/s41065-018-0058-4

Hereditas

Table 1 Amplification results of EST-SSR, SRAP, and SCoT primers

From: Genetic diversity and structure of tea plant in Qinba area in China by three types of molecular markers

Primer name	Sequence(5′-3′)	Total number of bands(TNB)	The number of polymorphic bands(NPB)	Percentage of polymorphic bands(PPB)%	Polymorphism information content(PIC)
SCoT1	CAACAATGGCTACCACCC	6	5	83.33	0.98
SCoT2	CAACAATGGCTACCACCG	10	10	100	0.98
SCoT3	CAACAATGGCTACCACCT	7	7	100	0.98
SCoT4	CAACAATGGCTACCACGA	11	11	100	0.84
SCoT5	CAACAATGGCTACCACGC	13	13	100	0.96
SCoT6	CAACAATGGCTACCACGT	10	10	100	0.89
SCoT7	CAACAATGGCTACCAGCA	13	12	92.31	0.88
SCoT8	CAACAATGGCTACCAGCC	12	11	91.67	0.95
SCoT9	ACGACATGGCGACCATCG	10	10	100	0.92
SCoT10	ACGACATGGCGACCACGC	8	8	100	0.93
SCoT11	ACCATGGCTACCACCGAC	9	9	100	0.87
SCoT12	ACCATGGCTACCACCGCC	13	12	92.31	0.90
SCoT13	ACCATGGCTACCACCGCG	12	10	83.33	0.87
SCoT14	ACGACATGGCGACCCACA	13	12	92.31	0.91
SCoT15	ACCATGGCTACCACCGGG	14	14	100	0.98
SCoT16	CCATGGCTACCACCGCCA	10	9	90	0.91
SCoT17	CCATGGCTACCACCGCAC	10	10	100	0.96
SCoT18	CCATGGCTACCACCGCAG	6	6	100	0.94
SCoT19	ACCATGGCTACCACCGCA	12	12	100	0.87
SCoT20	CATGGCTACCACCGGCCC	12	12	100	0.95
SCoT21	ACGACATGGCGACCAACT	9	9	100	0.93
SCoT22	ACGACATGGCGACCAACC	11	11	100	0.95
SCoT23	ACGACATGGCGACCATCC	6	6	100	0.77
SCoT24	ACGACATGGCGACCACGG	10	10	100	0.95
SCoT25	ACGACATGGCGACCACGT	13	12	92.31	0.89
SCoT26	ACCATGGCTACCACCGCT	10	10	100	0.97
SCoT27	ACCATGGCTACCACCGGA	9	9	100	0.90
SCoT28	CCATGGCTACCACCGCCC	13	13	100	0.77
SCoT29	CCATGGCTACCACCGCCG	13	11	100	0.89
SCoT30	CCATGGCTACCACCGGCT	4	4	100	0.92
SCoT31	CCATGGCTACCACCGCAA	10	10	100	0.89
SCoT32	CCATGGCTACCACCGCAT	7	7	100	0.98
SCoT33	CATGGCTACCACCGGCCA	17	17	100	0.87
SCoT34	CCATGGCGACCACCGGCA	11	10	90.91	0.71
SCoT35	CCATGGCGACCACCGGCG	17	15	88.24	0.81
SCoT36	CCATGGCGACCACCGGCC	14	13	92.86	0.68
SCoT37	CCATGGCGACCACCGCCG	16	16	100	0.74
SCoT38	CCATGGTCACCACCGGCG	13	12	92.31	0.56
SRAP1	Me1:TGAGTCCAAACCGGATA	8	8	100	0.79
SRAP1	Em6: GACTGCGTACGAATTCTA	8	8	100	0.79
SRAP2	Me1:TGAGTCCAAACCGGATA	7	7	100	0.87
SRAP2	Em10:GACTGCGTACGAATTCCA	7	7	100	0.87
SRAP3	Me1:TGAGTCCAAACCGGATA	8	8	100	0.82
SRAP3	Em13:GACTGCGTACGAATTTGA	8	8	100	0.82
SRAP4	Me2:TGAGTCCAAACCGGCTT	9	8	88.89	0.98
SRAP4	Em5:GACTGCGTACGAATTCTG	9	8	88.89	0.98
SRAP5	Me2:TGAGTCCAAACCGGCTT	8	7	87.5	0.94
SRAP5	Em9:GACTGCGTACGAATTCAT	8	7	87.5	0.94
SRAP6	Me2:TGAGTCCAAACCGGCTT	9	8	88.89	0.95
SRAP6	Em11:GACTGCGTACGAATTCAC	9	8	88.89	0.95
SRAP7	Me2:TGAGTCCAAACCGGCTT	10	8	80	0.85
SRAP7	Em14:GACTGCGTACGAATTCTT	10	8	80	0.85
SRAP8	Me3:TGAGTCCAAACCGGCTG	8	8	100	0.86
SRAP8	Em1:GACTGCGTACGAATTATC	8	8	100	0.86
SRAP9	Me3:TGAGTCCAAACCGGCTG	9	9	100	0.78
SRAP9	Em8:GACTGCGTACGAATTTCC	9	9	100	0.78
SRAP10	Me4:TGAGTCCAAACCGGCCA	12	10	83.33	0.95
SRAP10	Em1:GACTGCGTACGAATTATC	12	10	83.33	0.95
SRAP11	Me4:TGAGTCCAAACCGGCCA	11	10	90.9	0.98
SRAP11	Em2:GACTGCGTACGAATTTAT	11	10	90.9	0.98
SRAP12	Me4:TGAGTCCAAACCGGCCA	7	7	100	0.99
SRAP12	Em3:GACTGCGTACGAATTTAG	7	7	100	0.99
SRAP13	Me4:TGAGTCCAAACCGGCCA	8	8	100	0.96
SRAP13	Em7:GACTGCGTACGAATTTCT	8	8	100	0.96
SRAP14	Me5:TGAGTCCAAACCGGGTA	10	10	100	0.96
SRAP14	Em2:GACTGCGTACGAATTTAT	10	10	100	0.96
SRAP15	Me5:TGAGTCCAAACCGGGTA	10	9	90	0.93
SRAP15	Em3:GACTGCGTACGAATTTAG	10	9	90	0.93
SRAP16	Me5:TGAGTCCAAACCGGGTA	9	9	100	0.98
SRAP16	Em5:GACTGCGTACGAATTCTG	9	9	100	0.98
SRAP17	Me6:TGAGTCCAAACCGGTGA	11	10	90.9	0.92
SRAP17	Em2:GACTGCGTACGAATTTAT	11	10	90.9	0.92
SRAP18	Me6:TGAGTCCAAACCGGTGA	12	9	81.82	0.85
SRAP18	Em3:GACTGCGTACGAATTTAG	12	9	81.82	0.85
SRAP19	Me7:TGAGTCCAAACCGGTGT	13	11	84.62	0.95
SRAP19	Em4r:GACTGCGTACGAATTTGT	13	11	84.62	0.95
SRAP20	Me7:TGAGTCCAAACCGGTGT	12	11	91.67	0.84
SRAP20	Em9:GACTGCGTACGAATTCAT	12	11	91.67	0.84
SRAP21	Me7:TGAGTCCAAACCGGTGT	7	6	75	0.86
SRAP21	Em12:GACTGCGTACGAATTCAA	7	6	75	0.86
SRAP22	Me7:TGAGTCCAAACCGGTGT	7	7	100	0.92
SRAP22	Em14:GACTGCGTACGAATTCTT	7	7	100	0.92
SRAP23	Me8:TGAGTCCAAACCGGACC	5	5	100	0.96
SRAP23	Em2:GACTGCGTACGAATTTAT	5	5	100	0.96
SRAP24	Me8:TGAGTCCAAACCGGACC	11	8	72.73	0.94
SRAP24	Em4:GACTGCGTACGAATTTGT	11	8	72.73	0.94
SRAP25	Me8:TGAGTCCAAACCGGACC	10	8	80	0.95
SRAP25	Em8:GACTGCGTACGAATTTCC	10	8	80	0.95
SRAP26	Me8:TGAGTCCAAACCGGACC	8	7	87.5	0.94
SRAP26	Em13:GACTGCGTACGAATTTGA	8	7	87.5	0.94
SRAP27	Me9:CTTACTTAGACCGGAGT	7	6	85.71	0.97
SRAP27	Em2:GACTGCGTACGAATTTAT	7	6	85.71	0.97
SRAP28	Me9:CTTACTTAGACCGGAGT	9	7	77.78	0.97
SRAP28	Em3:GACTGCGTACGAATTTAG	9	7	77.78	0.97
SRAP29	Me9:CTTACTTAGACCGGAGT	5	5	100	0.98
SRAP29	Em5:GACTGCGTACGAATTCTG	5	5	100	0.98
SRAP30	Me9:CTTACTTAGACCGGAGT	6	6	100	0.99
SRAP30	Em6:GACTGCGTACGAATTCTA	6	6	100	0.99
SRAP31	Me9:CTTACTTAGACCGGAGT	7	6	85.71	1.00
SRAP31	Em9:GACTGCGTACGAATTCAT	7	6	85.71	1.00
SRAP32	Me9:CTTACTTAGACCGGAGT	8	7	87.5	0.90
SRAP32	Em14:GACTGCGTACGAATTCTT	8	7	87.5	0.90
SRAP33	Me10:TGAGTCCAAACCGGAAA	9	7	77.78	0.97
SRAP33	Em5:GACTGCGTACGAATTCTG	9	7	77.78	0.97
SRAP34	Me10:TGAGTCCAAACCGGAAA	8	8	100	0.96
SRAP34	Em7:GACTGCGTACGAATTTCT	8	8	100	0.96
SRAP35	Me10:TGAGTCCAAACCGGAAA	5	5	100	0.93
SRAP35	Em13:GACTGCGTACGAATTTGA	5	5	100	0.93
SRAP36	Me10:TGAGTCCAAACCGGAAA	8	6	75	0.99
SRAP36	Em14:GACTGCGTACGAATTCTT	8	6	75	0.99
SRAP37	Me11:GTACATAGAACCGGAGT	6	5	83.33	0.96
SRAP37	Em4:GACTGCGTACGAATTTGT	6	5	83.33	0.96
SRAP38	Me11:GTACATAGAACCGGAGT	6	5	83.33	1.00
SRAP38	Em5:GACTGCGTACGAATTCTG	6	5	83.33	1.00
SRAP39	Me11:GTACATAGAACCGGAGT	5	5	100	0.97
SRAP39	Em7:GACTGCGTACGAATTTCT	5	5	100	0.97
SRAP40	Me11:GTACATAGAACCGGAGT	10	8	80	0.98
SRAP40	Em14:GACTGCGTACGAATTCTT	10	8	80	0.98
EST-SSR2	F:GTCAAGAAAGCTCAAGGC	11	10	90.91	0.96
EST-SSR2	R:GATGGGCTTGTCTTCGTC	11	10	90.91	0.96
EST-SSR4	F:GTCAAGAAAGCTCAAGGC	7	7	100	0.96
EST-SSR4	R:TGTCTTGTGACCAAATTGAC	7	7	100	0.96
EST-SSR5	F:GTCAAGAAAGCTCAAGGC	6	6	100	0.89
EST-SSR5	R:TGAAGTGGCGGCGGAAGA	6	6	100	0.89
EST-SSR7	F:GTCAAGAAAGCTCAAGGC	5	5	100	0.97
EST-SSR7	R:GTCAAGTCAAAAACGCCG	5	5	100	0.97
EST-SSR9	F:CCACCGTTGATTCTACTTT	12	11	91.67	0.97
EST-SSR9	R:AACAGAGCATACCCAGAAG	12	11	91.67	0.97
EST-SSR14	F:CCACCGTTGATTCTACTTT	9	9	100	0.99
EST-SSR14	R:AAGACCCATACAAAAGATACT	9	9	100	0.99
EST-SSR15	F:CCACCGTTGATTCTACTTT	8	7	87.5	0.92
EST-SSR15	R:GATGGGCTTGTCTTCGTC	8	7	87.5	0.92
EST-SSR17	F:CCACCGTTGATTCTACTTT	9	9	100	0.89
EST-SSR17	R:GTCAAGTCAAAAACGCCG	9	9	100	0.89
EST-SSR19	F:CCACCGTTGATTCTACTTT	6	6	100	0.98
EST-SSR19	R:CTGCGAACCCTCTTGACC	6	6	100	0.98
EST-SSR20	F:ATCCACCGTATGATGCT	5	5	100	0.98
EST-SSR20	R:GATGGGCTTGTCTTCGTC	5	5	100	0.98
EST-SSR23	F:GAATCAGTGAATAAAGCGTGTA	8	8	100	0.97
EST-SSR23	R:TGAAGTGGCGGCGGAAGA	8	8	100	0.97
EST-SSR24	F:GAATCAGTGAATAAAGCGTGTA	7	7	100	0.99
EST-SSR24	R:TTGGTAGCCTCTTCTTTTG	7	7	100	0.99
EST-SSR26	F:CTCCGATTACTTTCTTCC	7	6	85.71	0.98
EST-SSR26	R:GATGACGATGGAGTGGG	7	6	85.71	0.98
EST-SSR27	F:CATAGTAGAGAAGACCACCA	8	7	87.5	0.99
EST-SSR27	R:GATGGGCTTGTCTTCGTC	8	7	87.5	0.99
EST-SSR30	F:CATAGTAGAGAAGACCACCA	7	6	85.71	0.94
EST-SSR30	R:GATGACGATGGAGTGGG	7	6	85.71	0.94
EST-SSR33	F:GAAAGTGCGAAACCAAAC	3	3	100	0.98
EST-SSR33	R:TGAAGTGGCGGCGGAAGA	3	3	100	0.98
EST-SSR35	F:GAAAGTGCGAAACCAAAC	6	6	100	0.93
EST-SSR35	R:GTCAAGTCAAAAACGCCG	6	6	100	0.93
EST-SSR38	F:GAAAGTGCGAAACCAAAC	9	9	100	0.94
EST-SSR38	R:CTGCGAACCCTCTTGACC	9	9	100	0.94
EST-SSR39	F:CAAGCAATACATACACACA	16	14	87.5	0.86
EST-SSR39	R:AAGACCCATACAAAAGATACT	16	14	87.5	0.86
EST-SSR43	F:CAAGCAATACATACACACA	5	5	100	0.98
EST-SSR43	R:AAAACAAGCCACCTCTA	5	5	100	0.98
EST-SSR47	F:CTCTTGATTGGTGCCTTTA	11	10	90.91	0.92
EST-SSR47	R:AAGACCCATACAAAAGATACT	11	10	90.91	0.92
EST-SSR49	F:CTCTTGATTGGTGCCTTTA	9	9	100	0.92
EST-SSR49	R:GATGGGCTTGTCTTCGTC	9	9	100	0.92
EST-SSR50	F:CTCTTGATTGGTGCCTTTA	7	7	100	0.98
EST-SSR50	R:TGTCTTGTGACCAAATTGAC	7	7	100	0.98
EST-SSR52	F:CTCTTGATTGGTGCCTTTA	12	11	91.67	0.82
EST-SSR52	R:TGAAGTGGCGGCGGAAGA	12	11	91.67	0.82
EST-SSR53	F:CATTGCCTTGATGCTGA	9	9	100	0.90
EST-SSR53	R:AAGACCCATACAAAAGATACT	9	9	100	0.90
EST-SSR56	F:CATTGCCTTGATGCTGA	9	9	100	0.91
EST-SSR56	R:GATGGGCTTGTCTTCGTC	9	9	100	0.91
EST-SSR58	F:CATTGCCTTGATGCTGA	12	10	83.33	0.98
EST-SSR58	R:TGAAGTGGCGGCGGAAGA	12	10	83.33	0.98
EST-SSR59	F:CATTGCCTTGATGCTGA	13	11	84.62	0.96
EST-SSR59	R:GTCAAGTCAAAAACGCCG	13	11	84.62	0.96
EST-SSR64	F:CCACCGTTGATTCTACTTT R:TGTCTTGTGACCAAATTGAC	8	8	100	0.89
EST-SSR67	F:CCACCGTTGATTCTACTTT	9	8	88.89	0.91
EST-SSR67	R:TGAAGTGGCGGCGGAAGA	9	8	88.89	0.91
EST-SSR69	F:ATCCACCGTATGATGCT	8	7	87.5	0.76
EST-SSR69	R:AAGACCCATACAAAAGATACT	8	7	87.5	0.76
EST-SSR72	F:ATCCACCGTATGATGCT	9	9	100	0.83
EST-SSR72	R:GTCAAGTCAAAAACGCCG	9	9	100	0.83
EST-SSR74	F:ATCCACCGTATGATGCT	9	8	88.89	0.88
EST-SSR74	R:TTGGTAGCCTCTTCTTTTG	9	8	88.89	0.88
EST-SSR76	F:GAATCAGTGAATAAAGCGTGTA	6	6	100	0.98
EST-SSR76	R:GATGGGCTTGTCTTCGTC	6	6	100	0.98
EST-SSR77	F:GAATCAGTGAATAAAGCGTGTA	7	6	85.72	0.94
EST-SSR77	R:GCAGGTTAGCGGTGGTTA	7	6	85.72	0.94
EST-SSR85	F:GAATCAGTGAATAAAGCGTGTA	6	6	100	0.94
EST-SSR85	R:GATGACGATGGAGTGGG	6	6	100	0.94
EST-SSR88	F:GAAAGTGCGAAACCAAAC	8	7	87.5	0.97
EST-SSR88	R:AAGACCCATACAAAAGATACT	8	7	87.5	0.97
EST-SSR91	F:GAAAGTGCGAAACCAAAC	5	5	100	0.95
EST-SSR91	R:TGTCTTGTGACCAAATTGAC	5	5	100	0.95
EST-SSR99	F:CAAGCAATACATACACACA	4	4	100	0.94
EST-SSR99	R:TTGGTAGCCTCTTCTTTTG	4	4	100	0.94
EST-SSR40	F:ATCCACCGTATGATGCT	5	5	100	0.98
EST-SSR40	R:TGTCTTGTGACCAAATTGAC	5	5	100	0.98

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ISSN: 1601-5223

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