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Table 4 EST-SSR results achieved in hybrid populations and their parents

From: Hybrid identification and genetic variation of Elymus sibiricus hybrid populations using EST-SSR markers

Population

Primer

T

M

TP

MF1

FF1

EF1

PPB (%)

1

Elw1420s081

3

0

3

0

1

0

100.00

Elw3545s194

3

3

0

3

3

0

0.00

Elw5627s404

14

0

14

3

2

6

100.00

Ps3577

4

2

2

2

2

1

50.00

Ps938

7

1

6

1

1

0

85.71

Ltc0055

6

3

3

3

3

0

50.00

Total

37

9

28

12

12

7

75.68

2

Elw1420s081

6

1

5

1

1

4

83.33

Elw3545s194

3

1

2

1

2

0

66.67

Elw5627s404

14

2

12

4

5

3

85.71

Ps1830

9

4

5

5

5

0

55.56

Ps2283

2

1

1

1

1

1

50.00

Ps3577

4

2

2

3

2

0

50.00

Ps938

5

2

3

5

2

0

60.00

Ltc0055

3

3

0

3

3

0

0.00

Ltc0157

11

3

8

4

3

1

72.73

Total

57

19

38

27

24

9

66.67

3

Elw1420s081

5

1

4

1

1

3

80.00

Elw3545s194

3

1

2

1

2

0

66.67

Elw5627s404

14

1

13

5

2

3

92.86

Ps1830

10

3

7

4

2

0

70.00

Ps2283

2

1

1

1

1

1

50.00

Ps3577

4

2

2

3

3

0

50.00

Ps938

5

1

4

1

2

0

80.00

Ltc0055

3

3

0

3

3

0

0.00

Ltc0157

11

3

8

3

4

1

72.73

Total

57

16

41

22

20

8

71.93

4

Elw3545s194

2

2

0

2

2

0

0.00

Elw5627s404

7

1

6

1

4

0

85.71

Ps1830

7

2

5

2

6

0

71.43

Ps2283

3

1

2

1

2

0

66.67

Ps3577

8

1

7

2

3

0

87.50

Ps938

6

0

6

0

1

0

100.00

Ltc0055

3

3

0

3

3

0

0.00

Ltc0157

5

1

4

1

2

0

80.00

Total

41

11

30

12

23

0

73.17

5

Elw1420s081

3

0

3

0

1

0

100.00

Elw3545s194

3

3

0

3

3

0

0.00

Elw5627s404

6

0

6

0

3

1

100.00

Ps1830

8

2

6

2

3

2

75.00

Ps2283

3

0

3

1

1

1

100.00

Ps3577

4

2

2

2

2

0

50.00

Ps938

4

1

3

1

2

2

75.00

Ltc0055

4

4

0

4

4

0

0.00

Ltc0157

8

3

5

3

5

1

62.50

Total

43

15

28

16

24

7

65.12

  1. T = Total number of amplified bands; M = Number of monomorphic bands; TP = Total number of polymorphic bands; MF1 = Bands shared by male parents and hybrids; FF1 = Bands shared by female parents and hybrids; EF1 = Bands exclusively present in hybrids; PPB = Percentage of polymorphic bands