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Table 1 Occurrence of posterior cross vein (PCV) in Dg knockdown flies, assayed together with 3 different genetic backgrounds

From: Drosophila exoribonuclease nibbler is a tumor suppressor, acts within the RNAi machinery and is not enriched in the nuage during early oogenesis

Genotype

PCV < 5%

PCV < 50%

PCV > 50%

Total wings

MS1096-GAL4 > Dg i/Y; ftl i/+

30.1%

16.5%

53.4%

402

MS1096-GAL4 > Dg i/Y; 3505-2a i/+

37.5%

7.6%

54.9%

662

MS1096-GAL4 > Dg i/Y; nbr i 37/+

14.9%

12.4%

72.7%

442

  1. Allelic classes are subdivided into 3 classes: those flies that show <5% PCV occurrence, those <50% PCV occurrence and those with >50% occurrence. As read out system, a MS1096-GAL4-driven Dg knockdown construct [13] was used. This construct creates loss of PCV to various extent (Fig. 4c). Lane 1, an unrelated ftl gene [8]. Lane 2, CG3505-2a i (unpublished data). Both unrelated genetic backgrounds were used as a negative controls. Lane 1 and 2 show that 54% of all flies have 50% or more PCV tissue present. Lane 3, in doubly ds-nbr; ds-Dg flies, this value increased to 73%, indicating that lowering nbr activity suppresses the knock-down activity on Dg, suggesting that nbr interferes with the RNAi machinery
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Hereditas

ISSN: 1601-5223

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