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Fig. 9 | Hereditas

Fig. 9

From: Deciphering the potential ability of RG108 in cisplatin-induced HEI-OC1 ototoxicity: a research based on RNA-seq and molecular biology experiment

Fig. 9

In auditory cells (HEI-OC1) cells, P-PI3K was down-regulated and P-AKT was down-regulated after cisplatin injury, but RG108 reversed this phenomenon. However, RG108 had no effect on the expression of PI3K and AKT. Caspase-3 was up-regulated after cisplatin injury, but RG108 reversed this phenomenon. A Western blotting was used to detect P-PI3K, P-AKT, PI3K and AKT protein levels in HEI-OC1 cells treated with DMSO, CIS, CIS + RG108, RG108. B Use ImageJ to analyze the relative expression of P-PI3K/PI3K protein. C Use ImageJ to analyze the relative expression of P-AKT/AKT protein. D Western blotting was used to detect Caspase-3 protein levels in HEI-OC1 cells treated with DMSO, CIS, CIS + RG108, RG108. E Use ImageJ to analyze the relative expression of Caspase-3 protein. Data are expressed as mean ± SD, n = 3,**** P < 0.0001, compared with the control group; Compared with cisplatin group, ### P < 0.001, #### P < 0.0001

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