Fig. 3
From: EZH2 regulates pancreatic cancer cells through E2F1, GLI1, CDK3, and Mcm4
RT-qPCR detected the expression level of EZH2 mRNA in the establishment of EZH2 overexpression and knockdown cell models. A and C showed the BXPC-3 cells, B and D showed the HPDE6-C7 cells. The internal reference target was β-actin gene. The value of 2-∆∆Ct represents the fold change in the expression of the target gene relative to the control group, with a value of 1 indicating no change in gene expression. *,**,*** represented significant differences between the different of EZH2 stable transfected cells models, P < 0.05, P < 0.01, and P < 0.001, respectively