Fig. 7
From: Circular RNA circHMCU promotes breast tumorigenesis through miR-4458/PGK1 regulatory cascade
MiR-4458 directly target PGK1. A The predicted binding site between miR-4458 and PGK1was exhibited. B The binding relationship between miR-4458 and PGK1 was verified by dual-luciferase reporter assay. C The expression of PGK1 in normal tissues (n = 66) and tumor tissues (n = 66) was tested by qRT-PCR. D The expression of PGK1 protein in in normal tissues and tumor tissues was tested by western blot. E PGK1 protein expression was measured in BC cell lines (MDA-MB-231 and MCF-7) and human normal mammary epithelial cell (MCF10A). F Pearson analysis was conducted to analyze the correlation between miR-4458 and PGK1 in BC tissues. G The expression of PGK1 protein was exhibited by western blot.*P < 0.05