Fig. 4
From: Circular RNA circHMCU promotes breast tumorigenesis through miR-4458/PGK1 regulatory cascade
CircHMCU was a sponge of miR-4458. A The predicted binding site between circHMCU and miR-4458 was exhibited. B and C The binding relationship between circHMCU and miR-4458 was verified by dual-luciferase reporter assay and RIP assay. D The expression of miR-4458 in normal tissues (n = 66) and tumor tissues (n = 66) was tested by qRT-PCR. E miR-4458 expression was measured in BC cell lines (MDA-MB-231 and MCF-7) and human normal mammary epithelial cell (MCF10A). F Pearson analysis was conducted to analyze the correlation between circHMCU and miR-4458 in BC tissues. *P < 0.05