Fig. 8

Relationship between risk score and microsatellite instability, tumor mutation load, and immune checkpoints. a Microsatellite instability. Patients with MSI-H had lower risk scores, whereas patients with COAD with MSI-L (P < 0.01) and MSS (P < 0.05) had higher risk scores. b Tumor mutational burden.The tumor mutational burden of subgroups with different risk scores was compared and the lower tumor mutation burden in the high-risk score group than in the low score group was observed (P < 0.05). c We further conducted significantly mutated gene (SMG) analysis of COAD samples from the low-and high-risk scoring subgroups. IPS for d PD1 alone and e PD1 combined with CTLA4 in the high and low scoring groups. The IPS of PD1 alone or in combination with CTLA4 was higher in the low-risk scoring group than in the high-scoring group (d-e). f Differences in CD274 expression between the high- and low-risk groups and CD274 expression was significantly higher in the low-risk group. *P < 0.05 and **P < 0.01, ***P < 0.001. IPS, immunophenotype score; SMG, significantly mutated gene