Fig. 5
Construction and validation of m6A methylation-related prognostic lncRNA prediction signature. a and b Least absolute shrinkage and selection operator (LASSO) analysis of 43 m6A methylation-associated prognostic lncRNAs in TCGA training cohort was performed, which revealed 13 prognostic predictive signatures: AC092944.1, AL137782.1, U91328.1, AC073896.3, ATP2B1-AS1, AL391095.2, SEPTIN7-DT, AC104819.3, LINC00861, AC003101.2, AC005229.4, AC156455.1, and AP001628.1. The patients were divided into high- and low-risk groups based on the median risk score. c and d Distribution of risk scores, OS status, and heat maps for 13 predictive m6A-associated lncRNAs in TCGA training cohort (c) and TCGA validation cohort (d). c-d The f risk scores, OS status, and expression profiles of the 13 prognostic predictive signatures of m6A methylation-associated lncRNAs in the training and validation cohorts. According to the heat map, the prognostic predictors in the high-risk group were significantly higher than those in the low-risk group, except for AL137782.1, AC104819.3, and AC073896.3, which showed lower expression levels than those in the low-risk group. e and f Kaplan–Meier curves of OS in patients with COAD according to risk scores in TCGA training cohort (e) and validation cohort (f). In TCGA training and validation cohorts, OS was longer in the low-risk group than in the high-risk group. g and h ROC curves for risk scores predicting 1-, 3-, and 5-year survival in TCGA training cohort (g) and TCGA validation cohort (h). In the training cohort, the 1-, 3-, and 5-year AUCs for the 13 prognostic factors were 0.787, 0.799, and 0.760, respectively (g). These values in the validation cohort were 0.749, 0.799, and 0.850, respectively (h). COAD, colon adenocarcinoma; LASSO, least absolute shrinkage and selection operator; lncRNA, long non-coding RNA; m6A: N6-methyladenosine; OS, overall survival; ROC, receiver operating characteristic; TCGA, The Cancer Genome Atlas