From: Transformation and gene-disruption in the apple-pathogen, Neonectria ditissima
Name | Sequence(5’→3’) | Description | |
---|---|---|---|
Set 1 | g47_Fw1 | atccagtaggccatcgttcc | Amplify NRPS AK830_g4721 and |
g47_Rv1 | ctggtgatgtgtccgtggat | Screen for knockouts | |
Set 2 | g47_Fw2 | ctttaccttacaatggcggcag | Amplify NRPS AK830_g4721 |
g47_Rv2 | gatcacaccagttcctcagca | ||
Set 3 | Backboneg47_Fw | ccattgacgactgtggtcattactgg | Amplify NRPS AK830_g4721 cloned in pJet1.2 |
Backboneg47_Rv | gaatcatgagtaaggggtactaactc | ||
Set 4 | Insertg47_Hyg_Fw | cccttactcatgattcgcacaaaaaaa | Amplify Hygromycin cassette |
Insertg47_Hyg_Rv | ccacagtcgtcaatgggtggagt | ||
Set 5 | T3 | gcaattaaccctcactaaagg | Amplify GFP/Hygromycin cassette from pCT74 |
T7 | taatacgactcactataggg |