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Table 2 Primers used in this study

From: Transformation and gene-disruption in the apple-pathogen, Neonectria ditissima

 

Name

Sequence(5’→3’)

Description

Set 1

g47_Fw1

atccagtaggccatcgttcc

Amplify NRPS AK830_g4721 and

g47_Rv1

ctggtgatgtgtccgtggat

Screen for knockouts

Set 2

g47_Fw2

ctttaccttacaatggcggcag

Amplify NRPS AK830_g4721

g47_Rv2

gatcacaccagttcctcagca

 

Set 3

Backboneg47_Fw

ccattgacgactgtggtcattactgg

Amplify NRPS AK830_g4721 cloned in pJet1.2

Backboneg47_Rv

gaatcatgagtaaggggtactaactc

 

Set 4

Insertg47_Hyg_Fw

cccttactcatgattcgcacaaaaaaa

Amplify Hygromycin cassette

Insertg47_Hyg_Rv

ccacagtcgtcaatgggtggagt

 

Set 5

T3

gcaattaaccctcactaaagg

Amplify GFP/Hygromycin cassette from pCT74

T7

taatacgactcactataggg