Table 2 Antibodies used for Western blotting

PI3 Kinase p85 (19H8) Rabbit mAb

CST

4257 T

1:1000

Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) (E3U1H) Rabbit mAb

CST

17366S

1:1000

AKT (pan) (C67E7) Rabbit mAb

CST

4691 T

1:1000

Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb

CST

4060S

1:2000

mTOR (7C10) Rabbit mAb

CST

2983 T

1:1000

Phospho-mTOR (Ser2448) Antibody

CST

2971S

1:1000

GAPDH (D16H11) XP® Rabbit mAb

CST

5174S

1: 1000

Anti-CDK1 (phospho T161) mAb

Abcam

ab201008-10 μl

1:1000

Anti-Cyclin D1 (CCND1) antibody

Abcam

ab40754-10 μl

1:1000

Anti-Raf1 antibody

Abcam

ab181115

1:1000

Anti-p27 KIP 1 (CDKN1B) antibody

Abcam

ab32034

1:1000

Anti-BTRC polyclonal Antibody

Solarbio

K002903p

1:500