Fig. 6

Arecoline treatment promotes cell cycle changes and affects apoptosis in HepG2 hepatoma cells. A-C Fraction of HepG2 hepatoma cells in the G1 A, S B, or G2 C phase after treatment with the indicated concentrations of arecoline. D Quantification of apoptosis in HepG2 cells. E Cell cycle in HepG2 cells on day 1, 3, 7 and con group. F Number of apoptotic cells after treatment with the indicated concentrations of arecoline, as assessed by annexin staining. Vs CON group, ^ap < 0.05; vs 1st day, ^bp < 0.05; vs3rd day, ^cp < 0.05