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Fig. 6 | Hereditas

Fig. 6

From: Inhibition of USP14 suppresses ferroptosis and inflammation in LPS-induced goat mammary epithelial cells through ubiquitylating the IL-6 protein

Fig. 6

Inhibition of NRF2 signaling pathway reversed the effect of IL-6 shRNA on ferroptosis of LPS-treated GMECs. LPS-treated GMECs were transfected with IL-6 shRNA alone or together treated with the NRF2 signaling protein inhibitor Brusatol. A-E The expression of NRF2 and its downstream pathway proteins were detected by Western blotting and the grayscale was analyzed by using ImageJ software. F Cell viability was detected by CCK-8 assay. G The secretion of TNF-α was detected by ELISA. H The content of ROS was analyzed with DCFDA-cell ROS detection kit. I MDA content was detected by MDA assay kit. J The GSH levels were detected by GSH assay kit. K The concentration of Fe2+ was measured by using an iron assay kit. L, M The expression level of GPX4 was detected by Western blotting and the grayscale was analyzed by using ImageJ software. N Represented images of transmission electron microscopy

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