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Fig. 4 | Hereditas

Fig. 4

From: Inhibition of USP14 suppresses ferroptosis and inflammation in LPS-induced goat mammary epithelial cells through ubiquitylating the IL-6 protein

Fig. 4

Overexpression of IL-6 reversed the effect of USP14 shRNA on ferroptosis of LPS-treated GMECs. LPS-treated GMECs were transfected with USP14 shRNA alone or together with CMV-IL-6. A-C The expression of USP14, IL-6, and GPX4 protein was detected by Western blotting and the grayscale was analyzed by using ImageJ software. D Cell viability was detected by CCK-8 assay. E The secretion of TNF-α was detected by ELISA. F The content of ROS was analyzed with DCFDA-cell ROS detection kit. G MDA content was detected by MDA assay kit. H The GSH levels were detected by GSH assay kit. I The concentration of Fe2+ was measured by using an iron assay kit

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Hereditas

ISSN: 1601-5223

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