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Fig. 3 | Hereditas

Fig. 3

From: Inhibition of USP14 suppresses ferroptosis and inflammation in LPS-induced goat mammary epithelial cells through ubiquitylating the IL-6 protein

Fig. 3

Effects of USP14 on IL-6 expression. A, B GMECs were treated with LPS, the expression level of USP14 protein was detected by Western blotting and the grayscale was analyzed by using ImageJ software. C-E LPS treated GMECs were transfected with CMV-USP14 expression vector or USP14 shRNA, the mRNA and protein expression levels of USP14 and IL-6 were detected by RT-qPCR and Western blotting, respectively. F The interaction between USP14 and IL-6 was verified by co-IP assay. G The effect of USP14 on the expression of IL-6 protein was detected by ubiquitin experiment

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Hereditas

ISSN: 1601-5223

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