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Fig. 2 | Hereditas

Fig. 2

From: Inhibition of USP14 suppresses ferroptosis and inflammation in LPS-induced goat mammary epithelial cells through ubiquitylating the IL-6 protein

Fig. 2

Effects of IL-6 on ferroptosis of LPS treated GMECs. LPS treated GMECs were transfected with CMV-IL-6 expression vector or IL-6 shRNA. A, B The overexpression and knockdown efficiency were detected by Western blotting and the grayscale was analyzed by using ImageJ software. A, C The expression level of GPX4 was detected by Western blotting and the grayscale was analyzed by using ImageJ software. D Cell viability was detected by CCK-8 assay. E The secretion of TNF-α was detected by ELISA. F The content of ROS was analyzed with DCFDA-cell ROS detection kit. G MDA content was detected by MDA assay kit. H The GSH levels were detected by GSH assay kit. I The concentration of Fe2+ was measured by using an iron assay kit

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