Fig. 4

SLC3A2 deficiency induces ferroptosis in HN13. A HN13-shNC and shSLC3A2 cells were treated with DMSO, Fer-1 (2.0 μM), Erastin (10.0 μM), Fer-1 (2.0 μM) + Erastin (2.0 μM), RSL3 (2.0 μM) and Fer-1 (2.0 μM) + RSL3 (2.0 μM), respectively, for 48 h and then cell viability was evaluated by MTT assay. In order to better show the difference of cell viability in different treated groups, we converted the cell viability of control group (treated with DMSO) to 100%, and then compare the cell viability of other groups. B&C The levels of total iron level and Fe²⁺ in laryngeal cancer cells were determined using iron assay kit. D The level of lipid ROS in laryngeal cancer cells was analyzed using cellular ROS assay kit. E The production of mitochondrial superoxide in laryngeal cancer cells was examined using MitoSOX™ Red Mitochondrial Superoxide Indicator. Results presented represent the means of triplicate experiments ± SEM. **p < 0.01