Fig. 5

Expression of hbn enhancer-Gal4 strains in the larval brain. A The genomic organization of the hbn locus is shown together with the locations of fragments from the upstream, intronic and downstream regions of the hbn locus used to test enhancer activities in the respective Gal4 strains. B-H Views of right hemispheres of Drosophila L3 larval brains. B‘-H‘ Views of the central brain focusing on the commissure region. An anti-Hbn antibody was used to visualize the nuclear Hbn expression pattern in blue, and enhancer-Gal4-driven UAS-H2B-mRFP1 (red) and/or UAS- mCD8::GFP (green) expression visualize the patterns generated by the various enhancers. In (C) and (H) an anti-Nrt antibody (green) was additionally used. The Gal4 strain numbers are indicated, yellow arrowheads indicate important regions showing coexpression of Hbn and the fluorescence marker, magenta arrowheads coexpression in primary neurons in (C) and the lamina in (G), red arrowheads indicate regions where only the enhancer expression is visible. Abbreviations are indicated in the figure. (Scale bars: 50 μm, B‘-H‘ as in B‘)