Fig. 2

Cell type identification of Hbn expressing cells in the larval brain. Images of larval brains showing the central brain regions (A, E) or brain hemispheres (B-D, F-L). A The earmuff R9D11 reporter (R9D11-mCD8-GFP, green) highlights the proximal parts of the six DM lineages (1–6) starting from the intermediate neural progenitor cells (INPs). DRx expression (blue) is observed in the more distal regions of each lineage compared with Hbn expression (red) in the more medial and proximal parts of these lineages. B A higher magnification image of some DM lineages shows that Hbn expression (red) in the proximal and medial regions is located more anteriorly (red arrowhead), and DRx expression (blue) in the medial and distal parts is located more posteriorly within the lineage (blue arrowhead). A region of coexpression of both proteins also exists (magenta arrowhead). C Dpn expression (green) marks INPs of type II lineages. Coexpression with Hbn (red) is observed in some cells of each lineage (yellow arrowheads). D The expression of Hbn (red) in combination with Pros (blue) and Elav (green) indicates Hbn expression in GMCs (Pros⁺, Elav⁻, white arrowhead) and neurons (Pros⁺, Elav⁺, yellow arrowhead). E Hbn (red) is not coexpressed with the glial cell marker Repo (green). F Hbn expression (green) overlaps with the DM time series marker Ey (blue) in some INPs (white arrowheads). G Toy (blue) is expressed more distally in neurons of the DM lineages and overlaps with Hbn (green) in a few cells (white arrowheads). (H-L) Sections of a larval brain hemisphere focusing on the lobula complex. Hbn expression (red) is shown in comparisons with dll-Gal4-mCD8::GFP (green). Abbreviations: DM, dorsomedial lineages; dIPC, dorsal inner proliferation centre; LP, lobula plate; ME, medulla; NP, neuropil. (Scale bars: 25 μm, 2B 10 μm)