Skip to main content
Fig. 5 | Hereditas

Fig. 5

From: TGF-β1/SMOC2/AKT and ERK axis regulates proliferation, migration, and fibroblast to myofibroblast transformation in lung fibroblast, contributing with the asthma progression

Fig. 5

Inhibition of AKT and ERK pathway reversed the promoting effect of SMOC2 overexpression on FMT. HBFs and IMR-90 cells were transfected with vector or SMOC2, and then treated with TGF-β1 or combined with PD98059 or LY294002. BrdU (A) and wound healing (B) assays were performed to assess cell proliferation and migration in HBFs and IMR-90 cells. **p < 0.01 compared with NT group; &&p < 0.01 compared with vector+TGF-β1 group; @@p < 0.01 compared with SMOC2 + TGF-β1 group. (C) The number of myofibroblasts in HBFs and IMR-90 cells was detected by flow cytometry analysis. **p < 0.01 compared with NT group; &&p < 0.01 compared with vector+TGF-β1 group; @@p < 0.01 compared with SMOC2 + TGF-β1 group. (D) The protein levels of collagen I and α-SMA in HBFs and IMR-90 cells were measured by western blot analysis. **p < 0.01 compared with NT group; &&p < 0.01 compared with vector+TGF-β1 group; @@p < 0.01 compared with SMOC2 + TGF-β1 group. (E) IF staining was performed to examine the protein content and distribution of α-SMA in HBFs and IMR-90 cells

Back to article page

Hereditas

ISSN: 1601-5223

Contact us