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Fig. 3 | Hereditas

Fig. 3

From: IFNAR1 gene mutation may contribute to developmental stuttering in the Chinese population

Fig. 3

In vitro cell signaling experiments of IFNAR1 variants using a dual-luciferase reporter system. HEK-293 cells were seeded in 24-well plates and then cotransfected with a plasmid DNA pGV208-hIFNAR1-WT or pGV208-hIFNAR1-MUT vector (e.g., pGV208-hIFNAR1-G301E, pGV208-hIFNAR1-K428Q, pGV208-hIFNAR1-L552P, or pGV208-hIFNAR1-del335) together with IFN-stimulated response element (ISRE) reporter and the pTK-Renilla Luc control vector. For the indicated groups, cells were incubated with IFN-β (100 IU/ml) for 12 h. After treatment, the cell lysates were examined to determine the ISRE reporter activity by using the dual-luciferase reporter assay system. *, P < 0.05; **, P < 0.01; ***, P < 0.001

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