Fig. 5

Phenotypic analysis of erm enhancer deletion strains. Laser confocal images of larval and adult brain hemispheres from erm enhancer deletion strains labelled with anti-Nrt to visualize secondary neurons and anti-DRx as a marker showing expression in the type II lineages, the mushroom bodies and in the optic lobe in larval brains and anti-Brp to visualize the adult brain. The right larval brain hemisphere and the right part of an adult brain is always shown. For all erm enhancer deletion strains homozygous animals were analysed. A Wild-type larval brain hemisphere showing the DRx expression in DM and DL lineages as well as in the optic lobe. B Strain R9D08^KO shows a more or less identical staining pattern relative to the wild-type strain. C In strain R9D10^KO, the brain hemisphere was enlarged and showed more lineages, and the DRx expression was increased throughout the hemisphere. D, E In strains R9D11^KO and R9D11S^KO the brain hemispheres were also enlarged and looked disorganized but showed less DRx staining than to R9D10^KO (C). F Right part of an adult brain of strain R9D10^KO showing an enlargement of the superior lateral protocerebrum (white arrowhead). Abbreviations: AL, antennal lobe; DL, dorsolateral lineages; DM, dorsomedial lineages; LH, Lateral Horn; OL, Optic Lobe; SOG, Suboesophageal Ganglion; SLP, Superior Lateral Protocerebrum; SMP, Superior Medial Protocerebrum. (Scale bars: 50 μm)