Fig. 2
From: Enhancer analysis of the Drosophila zinc finger transcription factor Earmuff by gene targeting
Expression of the ermKOGal4 strain. Laser confocal images showing the expression of the ermKOGal4 strain in different developmental stages visualized using an UAS-H2B-mRFP1 strain. A In a stage 16 embryo (the anterior end of the embryo is pointing down), ermKOGal4 dependent marker RFP expression is shown in red. Strong expression of the nuclear marker RFP was observed in the embryonic brain (red arrowhead). B In the right hemisphere of a third instar larval brain, Nrt staining (green) was used to highlight secondary neurons, ermKOGal4 marker expression in the type II lineages and the optic lobe is shown in red (red arrowheads). C The right part of an adult brain is visualized using Brp staining to mark the neuropile in green, and ermKOGal4 marker expression is again shown in red, here in the optic lobe and the central complex (red arrowheads). CX, central complex; DL, dorsolateral lineages; DM, dorsomedial lineages; OL, optic lobe. B’ larval brain with Nrt (red) and mCD8::GFP (green). C′ adult brain with Brp (red) and mCD8::GFP (green). (Scale bars: A, 25 μm; B-C′, 50 μm)