Fig. 6

Modulation of the Bcd gradient by Cyclin B. Pictures represent midsagittal confocal planes of embryos oriented with their dorsal side up and anterior to the left, except (C, H). A, B a nc 3 CycB RNAⁱ embryo stained for bcd mRNA (A) and DAPI (B). C, D, H a nc 1 CycB RNAⁱ embryo stained with mab YL_1,2 detecting freshly-made tubulin, and analyzed as a surface confocal Z-stack section (C) and a mid-sagittal confocal Z-stack of the same embryo (D), as well as a 3-D reconstruction combining multiple Z-stacks of the same embryo in (C, D) to reveal the microtubular pattern in the outermost 30 μm of the cortex. E, F mid-sagittal nc 2 CycB RNAⁱ embryo stained with Bcd antibody. Arrowhead denote filling of nuclei with Bcd. G heat-map of the intensities of Bcd protein concentrations in embryo (E) including scale from 0–255. Arrowhead denote filling of nuclei with Bcd. H surface 3-D reconstruction of the confocal stack used in (C, D) to demonstrate extensive formation of spontaneous asters, despite lack of MTOCs at the cortex. Stages of embryos are denoted in green and follow the nomenclature of [12]