Fig. 5

Modulation of the bcd gradient by an extra nuclear cycle. Pictures represent midsagittal confocal planes of embryos oriented with their dorsal side up and anterior to the left. A-C a nc 15 embryo from mh¹/mh¹ mothers stained for bcd mRNA(A, red), DAPI (B, blue) and the merge of (A-B) in (C). Note the double layer of nuclei due to an extra round of nuclear division, arrowheads in (B and N). D-I a nc 14 wild-type embryo stained for Bcd protein (D, green), Eve (F, red), Run (G, yellow), heat-map of the intensities of Bcd protein concentrations including scale from 0–255 (G), DAPI (H, blue) and the merge of (D-F, H) in (I). J-O nc 15 embryo from mh¹/mh¹ mothers stained for Bcd protein (J, green), Eve (K, red), Run (L, yellow), heat-map of the intensities of Bcd protein concentrations including scale from 0–255 (M), DAPI (N, blue) and the merge of (J-L, N) in (O)