Fig. 1

bcd RNA distribution in wispy mutants and expression of Wispy and PAP2 in embryos. A-E in situ hybridization of wisp^GK05287/Df(1)RA47 oocytes (A-B) and embryos (C-E) using bcd probes (in red) along with DAPI (in blue) to reveal nuclei. All panels are mid-sagittal single confocal sections mounted anterior end to the left and dorsal side up, where possible. In early staged oocytes, bcd mRNA is not transported to the anterior rim, rather it stays laterally in a stage 10 (A) or stage 14 oocyte (B). Consequently, in a wisp^GK0287/Df(1)RA47 embryo where growth is arrested during the first nuclear cycle (C), a deep cortical mRNA gradient is observed. In a freshly unfertilized wisp^GK0287/Df(1)RA47 embryo (D), a similar steep cortical gradient is observed as in (C), while in an old unfertilized wisp^GK0287/Df(1)RA47 embryo, the mRNA appears dispersed all over the anterior half. F-K single confocal sections on the apical surface of nuclear cycle 14 embryos, stained with anti-Wispy antibodies (F), DAPI staining (G) and the merge of both (H), anti-PAP2 antibodies (I), DAPI staining (J) and the merge of both (K). Note that both poly A polymerases stain the cytoplasm and are excluded from the nuclei