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Fig. 2 | Hereditas

Fig. 2

From: Nucleo-cytoplasmic shuttling of murine RBPJ by Hairless protein matches that of Su(H) protein in the model system Drosophila melanogaster

Fig. 2

Subcellular co-localization of RBPJ and H proteins. a Enlargements of salivary glands derived from homozygous RBPJwt larvae overexpressing the indicated H* protein isoform. Subcellular distribution of H protein is shown in green and of RBPJ protein in magenta; the left panel shows the merge. Size bar represents 50 μm in all panels. The following genotypes are depicted: sd-Gal4/+; RBPJwt / RBPJwt; UAS-Hcwt/+, sd-Gal4/+; RBPJwt / RBPJwt; UAS-H*NLS3/+, sd-Gal4/+; RBPJwt / RBPJwt; UAS-H*NES/+, sd-Gal4/+; RBPJwt / RBPJwt; UAS-H*NLS3*NES/+. b Nuclear to cytoplasmic (n/c) ratio is shown for H protein (green bars) and Su(H) protein (magenta bars), respectively, determined from 8 specimen each indicated as squares. Sample mean and standard deviation is indicated. The dotted line represents equal distribution in both compartments (i.e. nuclear equals cytoplasmic grey value). Hcwt is primarily nuclear, and H*NES even more enriched in nuclei. In contrast, H*NLS3 is located in the cytosol, whereas H*NLS3*NES is detected in the nuclear compartment as well. Note that RBPJwt strictly follows H* subcellular protein distribution. Statistical analysis was performed using ANOVA two-tailed Dunnett’s approach for multiple comparisons relative to the Hcwt control (*p < 0.05; **p < 0.01; ***p < 0.001)

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