Fig. 1

Crossing scheme. Crossing scheme for establishing (a) the driver line sd-Gal4; RBPJ^wt / CyO-GFP and (b) the effector lines RBPJ^wt / CyO-GFP; UAS-H* (representing the four different H alleles, UAS-H^cwt, UAS-H^*NLS3, UAS-H^*NES and UAS-H^*NLS3*NES, respectively). Direction of the cross is indicated with males (m), and virgin females (f). Note that sd-Gal4 is X-linked. Crosses of driver and effector lines result in the desired offspring, i.e. third instar larvae homozygous for RBPJ^wt that can be selected for the absence of the GFP marker for subsequent analysis of salivary glands