Table 1 Exome capture technology designs
From: Performance comparison of four types of target enrichment baits for exome DNA sequencing
Agilent | Twist | IDT | Dynegen | ||
|---|---|---|---|---|---|
Bait type | ssRNA | dsDNA | ssDNA | dsRNA | |
Bait length (bp) | 120 | 120 | 120 | 120 | |
Total bait length (MB) | 49.48 | 33.05 | 50.84 | 60.44 | |
Total target length (MB) | 35.7 | NP | 39.0 | NP | |
Method of library preparation | Dynegen | Twist | Dynegen | Dynegen | |
Fragmentation method | Ultrasonication | Enzymatic Fragmentation | Ultrasonication | Ultrasonication | |
DNA input for library preparation (ng) | 30 | 30 | 30 | 30 | |
%Exome covered | RefSeq(35.76 Mb) | 0.9533 | 0.9235 | 0.9357 | 0.9445 |
CCDS(32.28 Mb) | 0.9987 | 0.9967 | 0.9933 | 0.9924 | |
GENCODEv24(35.4 Mb) | 0.9943 | 0.9148 | 0.9344 | 0.9753 | |
KnownGene(36.75 Mb) | 0.9430 | 0.9098 | 0.9240 | 0.9503 | |